The rumours, which started about a week ago, also mentioned that Sofia Coppola would be directing the movie.
"Stop the madness," Longoria told People magazine. "Believe me, I would love to work with Beyonce one day. She's so talented. But this is definitely not something we are doing together. It's completely and absolutely not true."
The film was said to be an adaptation of Sarah Waters' book, "Tipping the Velvet."