He urged all regional states to support the ceasefire plan, adding that Russia will do whatever necessary to influence the Syrian leadership.
Calling on the Syrian opposition groups to stop military operations against Syrian government army and its supporting forces, Putin said, "We are counting on the United States to do the same with its allies and groups it supported."
"There was, finally, a real chance to end years of bloodshed and violence," Putin said.
Praising the joint intensive work done by Russian and US experts and negotiators, Putin said the ceasefire agreement could serve as an example for future responses to terrorist threats.
Putin also noted that the ceasefire agreement should facilitate humanitarian aid to all Syrian citizens.
"The main thing (of the agreement) is to create conditions for the launch of a genuine political process through a wide inclusive dialogue in Geneva under the auspices of the UN," Putin added.
Meanwhile, the president confirmed the continuation of strikes against the Islamic State, the Nusra Front and other terror groups.
Kremlin spokesman Dmitry Peskov on Monday said Russia would continue intense contacts on Syria issues with all interested countries, particularly the Middle East states.
"The main task now Russia and the US set after adoption of this joint statement is to stop unnecessary bloodshed and keep fighting terrorism, thus facilitate the political settlement in that country," Tass news agency quoted Peskov as saying.